Journal: British Journal of Pharmacology

Article Title: Androgen receptor antagonism accelerates disease onset in the SOD1 G93A mouse model of amyotrophic lateral sclerosis

doi: 10.1111/bph.14657

Figure Lengend Snippet: Effects of flutamide treatment on motor neuron and glial cell pathology in lumbar spinal cord ventral horns of SOD1G93A mice at postnatal day 120 (P120). (a) Representative immunostaining of ChAT‐positive motor neurons with (b) quantification. # P < 0.05, significantly different to male counterpart; two‐way ANOVA with Fisher's least significant difference test comparing sex effect. (c) Representative immunostaining of GFAP‐positive astrocytes with (d) quantification of astrocytes. # P < 0.05, significantly different to male counterpart; two‐way ANOVA with Fisher's least significant difference test comparing sex effect. (e) Representative immunostaining of CD11b‐positive microglia with (f) quantification of microglial cell counts. Data represent mean ± SEM, n = 5 mice per group. Scale bars = 50 μm

Article Snippet: Primary antibodies including rabbit anti‐androgen receptor (1:100 unpurified, Abcam, Cat# ab133273), goat anti‐ChAT (1:100, Millipore, Cat# AB144P, RRID:AB_2079751), rat anti‐GFAP (1:500, Zymed, Cat# 13‐0300), and rat anti‐CD11b (1:100, Bio‐Rad, Cat# MCA711, RRID:AB_321292) were prepared in 2% (v/v) normal donkey serum in 0.3% (v/v) Triton‐X 100 in 0.1‐M PBS for a single use 48‐hr incubation at 4°C.

Techniques: Immunostaining